MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody, Clone: [MGMT/8186R], Rabbit, Monoclonal
Artikelnummer:
NBT-4255-RBM8-P1ABX
Hersteller Artikelnummer:
4255-RBM8-P1ABX
Alternativnummer:
NBT-4255-RBM8-P1ABX-100
Hersteller:
NeoBiotechnologies
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IHC, WB
Spezies Reaktivität:
Human
Immunogen:
Recombinant fragment (around aa1-100) of human MGMT (exact sequence is proprietary)
Alternative Synonym:
6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross OOlinks at the O6 OO alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 OOposition of guanine in DNA (prior to cross OOlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA OOdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
Western Blot Analysis of MCF-7, LNCaP, Hep G2 and human liver lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8186R).
Western Blot Analysis of Pancreas tissue lysates of different species using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8186R).
Western Blot Analysis of Human Liver, Pancreas, Raji and MCF-7 lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8186R).
