Recognizes a 100kDa glycoprotein, identified as CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
SDS-PAGE Analysis of Purified CD10 Recombinant Rabbit Monoclonal Antibody (MME/8376R ). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney, Rabbit Kidney, Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine and Rabbit Intestine tissue lysates using CD10 Rabbit Recombinant Monoclonal Antibody (MME/8376R).
Western Blot Analysis of Human Kidney lysate using CD10 Rabbit Recombinant Monoclonal Antibody (MME/8376R).
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MME/8376R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
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