This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein.The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This MAb reacts with both normal and malignant epithelia of various tissues including breast and colon.
200ug/ml of Ab purified from Bioreactor Concentrate on Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistology (Formalin-paraffin) (0.5-1.0ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate bu
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV).
SDS-PAGE Analysis of Purified MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV). Confirmation of Integrity and Purity of Antibody
Flow Cytometric Analysis of PFA fixed MCF-7 cells. MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV), followed by goat anti-mouse IgG-CF488 (Blue), Isotype Control (Red)
Western Blot Analysis of human MCF-7 cell lysate using MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV).
Western Blot Analysis of Human kidney and Human Lung tissue lysates using MUC-1 Mouse Monoclonal Antibody (HMPV).
Western Blot Analysis of T47D cell lysate using MUC-1 Mouse Monoclonal Antibody (HMPV).
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