PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody, IgG2a, Clone: [PCNA/6580], Mouse, Monoclonal

Artikelnummer: NBT-5111-MSM5-P1
Artikelname: PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody, IgG2a, Clone: [PCNA/6580], Mouse, Monoclonal
Artikelnummer: NBT-5111-MSM5-P1
Hersteller Artikelnummer: 5111-MSM5-P1
Alternativnummer: NBT-5111-MSM5-P1-100
Hersteller: NeoBiotechnologies
Wirt: Mouse
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human
Immunogen: Recombinant full-length human PCNA protein
Alternative Synonym: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
Klonalität: Monoclonal
Klon-Bezeichnung: [PCNA/6580]
Molekulargewicht: 36kDa
Isotyp: IgG2a
NCBI: 5111
UniProt: P12004
Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Formalin-fixed, paraffin-embedded human tonsil stained with PCNA M
Formalin-fixed, paraffin-embedded human lymph nodestained with PCNA Mouse Monoclonal Antibody (PCNA/6580)at 2ug/ml. Inset: PBS instead of primary antibody, secondary only negative control
Formalin-fixed, paraffin-embedded human tonsil stained with PCNA Mouse Monoclonal Antibody (PCNA/6580)at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Western Blot Analysis of Raji cell lysate using PCNA Mouse Monoclonal Antibody (PCNA/6580).
Western Blot Analysis of Raji lysate using PCNA Mouse Monoclonal Antibody (PCNA/6580).
Western Blot Analysis of A-431, 293T, Hep G2 and NIH/3T3 cell lysates using PCNA Mouse Monoclonal Antibody (PCNA/6580).