Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
Western Blot Analysis of Raji lysate using PCNA Rabbit Recombinant Monoclonal Antibody (PCNA/8696R).
Western Blot Analysis of A-431, 293T, Hep G2 and NIH/3T3 cell lysates using PCNA Recombinant Rabbit Monoclonal Antibody (PCNA/8696R).
Western Blot Analysis of Intestine and Skin tissue lysates of different species using PCNA Recombinant Rabbit Monoclonal Antibody (PCNA/8696R).
Western Blot Analysis of Human Intestine,Human Skin, A549, HEK293, HeLa, Jurkat, K-562 and Raw lysates using PCNA Recombinant Rabbit Monoclonal Antibody (PCNA/8696R).
Formalin-fixed, paraffin-embedded human prostate stained with PCNA Recombinant Rabbit Monoclonal Antibody (PCNA/8696R). Inset: PBS instead of primary antibody, secondary only negative control.
SDS-PAGE Analysis of Purified PCNA Recombinant Rabbit Monoclonal Antibody (PCNA/8696R). Confirmation of Purity and Integrity of Antibody.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
