B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p
Recognizes a protein of 12kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml), Immunofluorescence (1-4ug/ml), Immunohistochemistry (Frozen) (1-2ug/ml for 30 minutes at RT)Optimal dilution for a specific application should be determined.
SDS-PAGE Analysis of Purified Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of human THP-1 and Raji cell lysate using Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1).
Immunofluorescent staining of HeLa cells. Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Flow Cytometric Analysis of PFA-fixed HeLa cells using Beta-2-Microglobulin Mouse Monoclonal Antibody (BBM.1), followed by goat anti-mouse IgG-CF488 (Blue), Isotype Control (Red).
Formalin-fixed, paraffin-embedded human kidney stained with Beta-2-microglobulin Mouse Monoclonal Antibody (BBM.1). Inset: PBS instead of primary antibody, secondary only negative control.
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