B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. This MAb reacts with all isoforms of CD45R expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes (Workshop IV). Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Formalin-fixed, paraffin-embedded human tonsil stained with CD45 Monoclonal Antibody (135-4C5).
Formalin-fixed, paraffin-embedded human tonsil stained with CD45 Monoclonal Antibody (135-4C5).
Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 Monoclonal Antibody (135-4C5) followed by Goat anti- Mouse- IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with CD45 Monoclonal Antibody (135-4C5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
SDS-PAGE Analysis of Purified CD45 MAb (135-4C5). Confirmation of Integrity and Purity of Antibody
Flow cytometry analysis of lymphocyte gated PBMCs unstained (gray) or stained with CF488A-labeled CD45 monoclonal antibody (135-4C5) (green).
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