B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
Formalin-fixed paraffin-embedded human tonsil stained with CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R).
Immunofluorescent analysis of PFA-fixed Raji cells. CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R) followed by goat anti-rabbit IgG-CF488 (green). Counterstain is phalloidin (red).
Flow Cytometric Analysis of PFA-fixed Raji cells. CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R) followed by goat anti-rabbit IgG-CF488 (blue), Isotype Control (red).
Western Blot Analysis of human spleen tissue lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783).
SDS-PAGE Analysis Purified CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of spleen tissue lysates of different species using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R).
Western Blot Analysis of human spleen tissue lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
