A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
Alternative Synonym:
Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
Western Blot An
Formalin-fixed, paraffin-embedded human non-Hodgkin&apos,s lymphoma stained with Bcl-2 Mouse Monoclonal Antibody (100/D5).
Intracellular staining of Bcl-2 in Jurkat cells. Bcl-2 Mouse Monoclonal Antibody (100/D5) (red), isotype control (green).
Western Blot Analysis of Bcl-2 in human skin tissue lysate using Bcl-2 Mouse Monoclonal Antibody (100/D5).
SDS-PAGE Analysis of Purified Bcl-2 Mouse Monoclonal Antibody (100/D5). Confirmation of Integrity and Purity of Antibody.
Western blot analysis of MCF-7 cell lysate using Bcl-2 Mouse Monoclonal Antibody (100/D5).
Western Blot Analysis of HDLM-2 lysate using bcl-2 Mouse Monoclonal Antibody (100/D5).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
