This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression ofbcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
SDS-PAGE Analysis of Purified Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of human MCF-7 cell lysate using Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).
Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/1878R).
Western Blot Analysis of Human Spleen, Human Skeletal Muscle, Jurkat, Raji and THP-1 lysates using BCL2 Recombinant Rabbit Monoclonal Antibody (BCL2/1878R).
Western blot analysis of Human Spleen, Mouse Spleen, Rat Spleen, Hamster Spleen, Guinea pig Spleen, Rabbit Spleen, Human Skeletal Muscle, Mouse Skeletal Muscle, Rat Skeletal Muscle, Hamster Skeletal Muscle, Guinea pig Skeletal Muscle, and Rabbit Skeletal Muscle tissue lysates using BCL2 Recombinant Rabbit Monoclonal Antibody (BCL2/1878R).
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