Replication Protein A2 (RPA2) Antibody, IgG1, Clone: [RPA2/4774], Mouse, Monoclonal
Artikelnummer:
NBT-6118-MSM4-P1ABX
Hersteller Artikelnummer:
6118-MSM4-P1ABX
Alternativnummer:
NBT-6118-MSM4-P1ABX-100
Hersteller:
NeoBiotechnologies
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC, WB
Spezies Reaktivität:
Human
Immunogen:
Recombinant human full-length Replication Protein A2 (RPA2)protein
Alternative Synonym:
p32, p34, REPA2, Replication protein A 32kDa subunit, Replication protein A 34kDa subunit, Replication protein A2, RF-A protein 2, Rf-A2, RFA, RP-A p32, RP-A p34, RP21C, RPA32, RPA34, RpLP1, RpP2
Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 2
Formalin-fixed, paraffin-embedded human tonsil s
Formalin-fixed, paraffin-embedded human colon stained with RPA2 Mouse Monoclonal Antibody (RPA2/4774).
Formalin-fixed, paraffin-embedded human colon stained with RPA2 Mouse Monoclonal Antibody (RPA2/4774).
Formalin-fixed, paraffin-embedded human tonsil stained with RPA2 Mouse Monoclonal Antibody (RPA2/4774).
SDS-PAGE Analysis Purified RPA2 Mouse Monoclonal Antibody (RPA2/4774). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific RPA2 Mouse Monoclonal Antibody (RPA2/4774). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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