Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkins disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Western Blot Analysis of HeLa cell lysate Fascin-1 Mouse Monoclonal Antibody (SPM133)
Formalin-fixed, paraffin-embedded human Hodgkin&apos,s Lymph stained with Fascin-1 Mouse Monoclonal Antibody (SPM133)
Western Blot Analysis of HeLa cell lysate Fascin-1 Mouse Monoclonal Antibody (SPM133)
SDS-PAGE Analysis of Purified Fascin-1 Mouse Monoclonal Antibody (SPM133). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of PFA-fixed K562 cells using Fascin-1 Mouse Monoclonal Antibody (SPM133) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red)
Immunofluorescence Analysis of HeLa cells labeling Fascin with Fascin-1 Mouse Monoclonal Antibody (SPM133)- IgG-CF640 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red).
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