Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin (TM) and spectrin A°a are present in erythrocytes, whereas spectrin (TM) II (also designated fodrin (TM)) and spectrin A°a I (also designated fodrin A°a) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin A°a III is highly homologous to both spectrin A°a I and spectrin A°a II. Spectrin A°a III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C
Formalin-fixed, paraffin-embedded human pancreas stained with Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778).
SDS-PAGE Analysis Purified Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human PANC-1 cell lysate using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778).
Western Blot Analysis of HeLa, PC-3 cell lysates and brain tissue lysate using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778).
Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling Spectrin beta III with Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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