TATA-box binding protein (TBP) interactions with TBP-associated factors (TAFs) are required for the transcription of RNA polymerases. One particular TBP-TAF complex, snRNA-activating protein complex (SNAPC), is unusual in that it regulates basal transcription of both RNA polymerase II and III by binding specifically to a non-TATA-box proximal sequence element (PSE). SNAPC consists of five subunits of varying size. SNAPC binds to Oct-1 and TBP, which are activators of snRNA and RNA polymerases, respectively. The POU domain of Oct-1 binds to SNAPC 190 and effectively recruits SNAPC to the PSE. The cooperative binding of SNAPC and Oct-1 to their respective sequence elements is mediated by a nucleosome positioned between the two sequence elements. SNAPC 19 mediates the assembly of the subunits to form a functional SNAPC transcription regulator. SNAPC 50 (also designated PTF) contains two zinc finger motifs and binds to SNAPC 43 (also designated PTF) but not SNAPC 45 (PTF).
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Western Blot Analysis of human HeLa cell lysate using TFAP2A Mouse Monoclonal Antibody (PCRP-TFAP2A-2C2).
Flow Cytometric Analysis of PFA-fixed HeLa cells. TFAP2A Mouse Monoclonal Antibody (PCRP-TFAP2A-2C2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
SDS-PAGE Analysis of Purified TFAP2A Mouse Monoclonal Antibody (PCRP-TFAP2A-2C2). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human HeLa cell lysate using TFAP2A Mouse Monoclonal Antibody (PCRP-TFAP2A-2C2).
Analysis of Protein Array containing more than 19,000 full-length human proteins using TFAP2A Mouse Monoclonal Antibody (PCRP-TFAP2A-2C2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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