This antibody recognizes both Vinculin (125kDa) and meta-Vinculin (135kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and (TM)-Actinin. In addition, vinculin, Talin and (TM)-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. (TM)-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
SDS-PAGE An
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with VCL-Monospecific Mouse Monoclonal Antibody (VCL/3617).
Formalin-fixed, paraffin-embedded human testis stained with VCL-Monospecific Mouse Monoclonal Antibody(VCL/3617).
SDS-PAGE Analysis VCL-Monospecific Mouse Monoclonal Antibody(VCL/3617). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Mouse Vinculin Monoclonal Antibody (VCL/3617). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Flow Cytometric Analysis of PFA-fixed U87 cells using Vinculin Mouse Monoclonal Antibody (VCL/3617) followed by goat anti-mouse IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescence Analysis of PFA fixed U87 cells labeling VCL-Monospecific Mouse Monoclonal Antibody (VCL/3617) followed by Goat anti-mouse IgG-CF488 (Green).
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