Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), ,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilut
SDS-PAGE
Formalin-fixed, paraffin-embedded human Uterus stained with Caldesmon Rabbit Recombinant Monoclonal Antibody (CALD1/1424R).
Formalin-fixed, paraffin-embedded Rat Uterus stained with Caldesmon Rabbit Recombinant Monoclonal Antibody (CALD1/1424R).
SDS-PAGE Analysis of Purified Caldesmon Rabbit Recombinant Monoclonal Antibody (CALD1/1424R). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of human ovary tissue lysate using Caldesmon Rabbit Recombinant Monoclonal Antibody (CALD1/1424R).
Western Blot analysis of Human Skeletal Muscle, Human Ovary, and HeLa lysates using CALD1 Recombinant Rabbit Monoclonal Antibody (CALD1/1424R).
Western Blot Analysis of Human Ovary, Mouse Ovary, Rat Ovary, Hamster Ovary, Guinea Pig Ovary and Rabbit Ovary tissue lysates using Caldesmon Recombinant Rabbit Monoclonal Antibody (CALD1/1424R).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
