Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (0.5-1ug/million cells), ,Immunofluorescence (1-2ug/ml), ,Western Blotting (1-2ug/ml for 60 minutes at RT),,Optimal dilution for a specific application should be determined.
Western Blot Analysis of human
Western Blot Analysis of human Thymus lysate using Purified CD3e Mouse Monoclonal Antibody (PC3/188A).
SDS-PAGE Analysis Purified CD3e Mouse Monoclonal Antibody (PC3/188A). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of human Jurkat Cell lysate using CD3e Mouse Monoclonal Antibody (PC3/188A).
Western Blot Analysis of human Jurkat Cell lysate using CD3e Mouse Monoclonal Antibody (PC3/188A).
Western Blot analysis of Jurkat cell lysate using CD3e Mouse Monoclonal Antibody (PC3/188A).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
