APY, ATOPY, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, Leukocyte surface antigen Leu-16, Ly44, MS4A1, MS4A2
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min foll
Flow Cytometric Analysis of Raji cells .CD20 M
Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (IGEL/773).
Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (IGEL/773).
Flow Cytometric Analysis of Raji cells .CD20 Mouse Monoclonal Antibody (L26), followed by goat anti-mouse IgG-CF488 (Blue), Isotype Control (Red).
SDS-PAGE Analysis of Purified CD20 Mouse Monoclonal Antibody (IGEL/773). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by goat anti-mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
