Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkins disease.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95&
Western Blot Analysis of Daudi cell lysate using CD86 Mouse Monoclonal Antibody (C86/1146).
Confocal Immunofluorescence image of Raji cells using CD8
Formalin-fixed, paraffin-embedded human Melanoma stained with CD86 Mouse Monoclonal Antibody (C86/1146).
Western Blot Analysis of Daudi cell lysate using CD86 Mouse Monoclonal Antibody (C86/1146).
Confocal Immunofluorescence image of Raji cells using CD86 Mouse Monoclonal Antibody (C86/1146) followed by goat anti-Mouse IgG conjugated with CF488 (green). Red Dot is used to label the Nuclei (red).
SDS-PAGE Analysis of Purified CD86 Mouse Monoclonal Antibody (C86/1146). Confirmation of Integrity and Purity of Antibody.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
