Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkins disease.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixe
Formalin-fixed, paraffin-embedded human Colon stained with CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).
Immunofluorescent Staining of Raji cells using CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R) followed by goat anti-rabbit IgG conjugated with CF488 (green). Nuclei are stained with Red Dot.
SDS-PAGE Analysis of Purified CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).
Western Blot Analysis of Ramos cell lysate using CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).
Flow Cytometric Analysis of Ramos cells using CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
