A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)
Alternative Synonym:
Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
ELISA (For coating, order antibody without BSA), Western Blot (1-2ug/ml for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
SDS-PAGE Analysis Purifi
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD47 Mouse Monoclonal Antibody (IAP/2937).
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD47 Mouse Monoclonal Antibody (IAP/2937).
SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody (IAP/2937). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of U87, THP-1 cell lysate using CD47 Mouse Monoclonal Antibody (IAP/2937).
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD47 Mouse Monoclonal Antibody (CD47/2937) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDis) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDis) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot analysis of Jurkat and Prostate cell lysate using CD47 Mouse Monoclonal Antibody (CD47/2937).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
