The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rat serum, as teste
Klonalität:
Monoclonal
Klon-Bezeichnung:
[Polyclonal]
Puffer:
Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins adde
Quelle:
Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Formel:
Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins add
Antibody Type:
Secondary Antibody
Anwendungsbeschreibung:
Precipitation assays. In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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