The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. In immunoelectrophoresis against swine serum, a single characteristic precipitin line is usually observed, representing primarily Igg2. The IgG1 may
Klonalität:
Monoclonal
Klon-Bezeichnung:
[Polyclonal]
Puffer:
Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum
Quelle:
Purified normal IgG isolated from pooled swine serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Formel:
Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat seru
Antibody Type:
Secondary Antibody
Anwendungsbeschreibung:
Precipitation assays. In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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