Mouse anti CD68 (Macrosialin), conjugated to FITC, IgG1, Clone: [Ki-M7], Monoclonal
Artikelnummer:
NMB-GM-4152
Hersteller Artikelnummer:
GM-4152
Alternativnummer:
NMB-GM-4152
Hersteller:
NordicMubio
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
FC
Spezies Reaktivität:
Human
Konjugation:
FITC
CD68 (macrosialin) is a type-one membrane protein with significant sequence homology to a family of lysosomal-associated glycoproteins (lamp). CD68 molecules are predominantly located intracellularily in cytoplasmic granules but can also be detected in s
Permeabilization and Staining Procedure - In combination with our Permeabilization Kit FIX&PERM? (Cat. No. GAS-002) intracellular CD68 can be easily stained in cell suspensions. - For each sample to be analyzed add 50 µl of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube - Add 100 µl of Reagent A (Fixation Medium, stored and used at room temperature) - Incubate for 15 minutes at room temperature - Add 5 ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g - Remove supernatant and add to cell pellet 100µl Reagent B (Permeabilization Medium) and 20 µl of the CD68 monoclonal antibody conjugate - Vortex at low speed for 1-2 seconds - Incubate for 15 minutes at room temperature - Wash cells with phosphate buffered saline as described above - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 % formaldehyde and store them at 2-8C in the dark. Analyze fixed cells within 24 hours
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