Ep-CAM is a 34 kD glycoprotein and can be detected at the basolateral membrane of the majority of epithelial tissues. It is intricately linked with the cadherin-catenin complex and hence the fundamental WNT pathway responsible for intracellular signallin
Each vial contains 100µg 1mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Quelle:
HEA125 is a mouse monoclonal IgG1 antibody derived by fusion of Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse cells immunized with the HT-29 colon carcinoma cell line.
Reinheit:
ProtG affinity chromatography
Anwendungsbeschreibung:
The HEA125 antibody is suitable for the detection of human EpCAM by Western blotting (under reducing and non-reducing conditions), flow cytometry and immunocytochemistry. The antibody is also suitable for the detection of human EpCAM by immunohistochemistry on frozen and paraffin embedded tissues. For the latter application, pre-treatment with pronase 0.1% or unmasking fluid C (Art. No. DE000) is recommended. Optimal antibody dilutions for the different applications should be determined by titration. For immmunohistochemistry a dilution of 1:100 to 1:500 is recommended. The monoclonal antibody HEA125 shows a broad reactivity pattern with most human epithelial cells and epithelium derived tumours. It doesnt react with epidermal keratinocytes, parietal cells, hepatocytes, thymus cortical epithelium, myoepithelia, and non-epithelial tissues.
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