A portion of amino acids 399-428 from the macfa protein was used as the immunogen for this MeCP2 antibody.
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. [RefSeq].
The stated application concentrations are suggested starting amounts. Titration of the MeCP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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