A portion of amino acids 55-83 from the human protein was used as the immunogen for the HBE1 antibody.
The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I, two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5-epsilon - G-gamma - A-gamma - delta - beta-3
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Anwendungsbeschreibung:
The stated application concentrations are suggested starting points. Titration of the HBE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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