Amino acids SADYNREHGGPEGMDPDGVIESNWNEI of human EIF4A2 were used as the immunogen for the EIF4A2 antibody.
Eukaryotic initiation factor 4A-II is a protein that in humans is encoded by the EIF4A2 gene. It is mapped to 18p11.2. Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice, yeast cells also possess two EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation.
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reinheit:
Antigen affinity
Formulierung:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Antibody Type:
Primary Antibody
Application Verdünnung:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Immunoprecipitation: 2ug per 500ug of lysate
Anwendungsbeschreibung:
Optimal dilution of the EIF4A2 antibody should be determined by the researcher.
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