Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA from the C-terminus were used as the immunogen for the Myc antibody.
Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
Anwendungsbeschreibung:
Optimal dilution of the Myc antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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