All Lanes: FAK Antibody at 1/ 2000 dilution.Lane1:HeLa cell lysate.Proteins at 20µg per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 119kDaObserved band size: 119kDa
Immunohistochemical analysis of paraffin-embedded mouse spleen, using FAK Antibody.Tris-EDTA Buffer (pH9.0).
Immunofluorescent analysis of Hela cells, using FAK Antibody.4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.
All Lanes: FAK Antibody at 1/ 2000 dilution.Lane1:MCF-7.Proteins at 20µg per laneLane2:SH-SY5Y.Proteins at 20µg per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 119kDaObserved band size: 119kDa
All Lanes: FAK Antibody at 1/ 2000 dilution.Lane1:A549.Lane2:NCI-H1299.Lane3:HUVEC.(Proteins at 20µg per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 119kDaObserved band size: 119kDa
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