All Lanes: Ephrin B2 Antibody at 1/ 1000 dilution.Lane1:HeLa.Lane2:293.Lane3:MCF-7.Lane4:K562.Lane5:U-87 MG.(Proteins at 20µg per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 50kDaObserved band size: 50kDa
All Lanes: Ephrin B2 Antibody at 1/ 1000 dilution.Lane1: HEK293 cell lysate.Lane2:Mouse spleen lysate.(Proteins at 20µg per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 50kDaObserved band size: 50kDa
All Lanes: Ephrin B2 Antibody at 1/ 1000 dilution.Lane1:K562.Proteins at 20µg per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 50kDaObserved band size: 50kDa
All Lanes: Ephrin B2 Antibody at 1/ 1000 dilution.Lane1:Mouse kidney.Lane2:Mouse brain.Lane3:Rat kidney.Lane4:Rat brain.(Proteins at 20µg per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 50kDaObserved band size: 50kDa
Immunofluorescent analysis using the Antibody at 1:50 dilution.4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.
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