0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Quelle:
Human
Formulierung:
Lyophilized
Anwendungsbeschreibung:
Human transferrin rhodamine conjugation has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing vari
Colocalization of ATP7B with TGN46 or LAMP1.Cells were left untreated (basal medium), treated with 10µM TTM (low copper) or treated with 10, 100 or 200µM CuCl2. (A,B) Merged images show ATP7B in green, TGN46 (A) or LAMP1 (B) in magenta and the nucleus in blue, pixel overlap is shown in white. Fig. 1. PMID: 31515276.
Dot Blot of Rhodamine Conjugated Human Transferrin. Dotted directly with Rhodamine Conjugated Human Transferrin at following concentrations. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng Primary antibody: none Secondary antibody: none Block: MB-070 for 1 HR at RT.
COMMD1-PtdIns(4,5)P2interaction measured by fluorescence quenching of all aromatics. (C) Example images showing colocalization of ATP7B with wild type, T174M and K167/173E COMMD1. ATP7B is in green and COMMD1 variants in magenta as indicated. Fig. 6.. PMID: 31515276.
Rab5c over activation leads to excessive endolysosomal trafficking of Notch ligands and receptor.(A) TRITC-conjugated TF internalization assay in Hela cells transfected with control empty pCS2 or pCS2-rab5cCA plasmid. Representative pictures were shown. Scale bar, 10 µm. (B) Quantitative fluorescence intensity of intracellular TRITC-TF in control empty pCS2 and pCS2-rab5cCA transfected cells,n= 8 cells for each group. Error bars, mean SD.Pvalue was calculated by Studentttest, ***P< 0.001. CA, constitutively active, TF, transferrin, TRITC, tetramethylrhodamine. S6 Fig. PMID: 32275659.
Rab5c function is in an EC autonomous manner.(A) TRITC-conjugated TF internalization assay in Hela cells transfected with empty pCS2 or pCS2-rab5cDN plasmids. Representative pictures were shown. Scale bar, 10 µm. (B) Quantitative fluorescence intensity of intracellular TRITC-TF in empty pCS2 or pCS2-rab5cDN transfected Hela cells,n= 8 cells for each group. Error bars, mean SD.Pvalue was calculated by Studentttest, ***P< 0.001. DN, dominant-negative, TF, transferrin, TRITC, tetramethylrhodamine. S3 Fig. PMID: 32275659.
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