0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Quelle:
Rabbit
Formulierung:
Lyophilized
Application Verdünnung:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Rabbit IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
SDS-PAGE of Rabbit IgG Whole Molecule. Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5µL OPAL Pre-stained Marker (MB-210-0500). Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1µg per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.
Lateral flow test results for 58 serum samples, including 51 normal and 7 positive samples. [Symbol legend: (*)P< 0.05, (****)P< 0.0001 (one-way analysis of variance and Fishers least significant difference test).] Mouse anti-human IgG antibody and rabbit IgG [p/n 011-0102] were combined, the mixture was dispensed onto the conjugate pad and dried. Figure 4. PMID: 32323974.
Representative sequences of the 15 identified groups from the NGS pool were screened for their individual binding abilities to Protein A. Comparative SPR-based interaction analyses were performed with the Biacore X100 instrument. Biotinylated aptamers were immobilized via the 5'-end (A) or 3'-end (B) on the streptavidin-modified sensor surface and 1000 nM Protein A was injected for binding. The sensor responses from the end of the binding phases (after 300 s) are shown. In addition, cross-specificities to other proteins were analyzed. Asterisks indicate if certain interactions have not been investigated. Rabbit IgG (p/n 011-0102). Figure 5. PMID: 29495282.
SDS PAGE gel of commercially-purified rabbit Immunoglobulin G (rIgG) and of rIgG papain digestion products Fab and Fc. Lanes A, B and G contain gel markers. Lanes C-F contain products which have been reduced with b-mercaptoethanol. Lanes H-L contain non-reduced products. Each lane contains approximately 35 mg of the following products: Lanes C and H contain papain digested products, Lanes D, I and L contain Fc fragment obtained from protein A purification, Lanes E and J contain whole rIgG, Lanes F and K contain Fab fragment obtained from protein A purification, Whole rIgG (p/n 011-0102), rFc (p/n 011-0103), rFab (p/n 011-0105), rF(ab)2 (p/n 011-0104). Fig. 3. PMID: 12643976.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
