This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 2 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-Notch2 antibody, AGS2 antibody, hN2 antibody, Notch homolog 2 antibody, neurogenic locus notch homolog protein 2
Anti-Notch2 has been tested in ELISA, WB, and IHC. Anti-NOTCH-2 has a strong response was detected by ELISA against the immunizing peptide. This product was assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated ant
Western Blot of Rabbit Anti-Notch 2 N-Term Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HeLa Whole Cell Lysate (p/n W09-000-364). Lane 3: MOLT-4 Whole Cell Lysate (p/n W09-001-GK4). Lane 4: K-562 Whole Cell Lysate (p/n W09-001-GJ7). Lane 5: Human Kidney Lysate. Load: 20µg lysate/lane. Primary Antibody: Anti-NOTCH 2 at 1:1000 for 1 hr at RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 30mins at RT. Blocking: 5% BLOTTO. Expected MW: ~86kDa.
Western Blot of Rabbit anti-NOTCH2 (Cleaved N terminal) antibody. Lane 1 Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 2: U-251 Lysate (p/n W09-001-GY4). Lane 3: PC-3 Lysate (p/n W09-001-GV6). Lane 4: MCF-7 Lysate (p/n W09-000-360). Lane 5: Hep-G2 Lysate (p/n W09-001-GJ5). Lane 6: A431 Lysate (p/n W09-000-361). Lane 7: A549 Lysate (p/n W09-001-372). Lane 8: J774A.1 Lysate (p/n W10-001-GX3). Lane 9: C2C12 Lysate (p/n W10-001-GL7). Lane 10: Mouse Embryonic Fibroblast Lysate (p/n W10-001-371). Lane 11: NIH/3T3 Lysate (p/n W10-000-358). Lane 12: PC-12 Lysate (p/n W12-001-GL9). Lane 13: Rat Brain Lysate (p/n W12-000-T077). Lane 14: Mouse Brain Lysate (p/n W10-000-T004). Lane 15 Marker: Opal Pre-stained Ladder. Load: 10 µg of lysate/lane. Primary antibody: NOTCH2 antibody at 1µg/mL overnight at 4C. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted size: ~86kDa in human cell lines (2-7) for NOTCH2.
Rocklands anti-NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Western blot Rabbit Anti-NOTCH 2 (Cleaved N terminal) Antibody. Negative Control: human pluripotent stem cells (lanes 1 and 2), definite endoderm cells (lanes 3 and 4), Positive Control: pancreatic endoderm cells (lanes 5 and 6), pancreatic progenitors cells (lanes 7 and 8). Primary Antibody: Anti-NOTCH 2 used at a 1:1000 dilution overnight at 4C. Vinculin served as loading control at 1:1000 at RT for 1hr. Secondary Antibody: donkey anti-rabbit HRP conjugated antibody or donkey anti-mouse HRP conjugated antibody diluted 1:5000 for 1h at RT. Strong unspecific bands. Nevertheless, a band over 250kDa appears to be highly specific, as it is only detected at day 10 (lanes 5 and 6) and day 14 (lanes 7 and 8). Independently Validated byantibodies-online GmbH (p/n ABIN1043688/ ABIN104899) courtesy of Ulm University Hospital.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
