This polyclonal antibody reacts with human ZBP-89 in a variety of tested immunological assays including western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a
Anti-ZBP-89 antibody used to confirm siRNA knockdown of ZBP-89. See Bai and Merchant (2003) for additional details.
ZBP-89 interacts with p53 in vivo and in vitro. ZBP-89 interacts with amino acids 160 to 393 of p53. p53 contains an activation domain (AD), DNA binding domain (DNA BD), tetramerization domain (TD), and regulatory domain (RD). p53 binds to the zinc finger DNA binding domain of ZBP-89 (amino acids 154 to 300). ZBP-89 contains an acidic domain (AD), zinc finger DNA binding domain, basic domain (BD), and C-terminal domain (C-TER). See Bai and Merchant (2001) for additional details.
Schematic diagram of ZBP-89 protein domains and function.
Serum starvation induces ZBP-89 and p53 expression. AGS (gastric carcinoma) cells were cultured in serum-free F-12 medium for the indicated times, and western blots were used to detect the expression profiles of ZBP-89, p53, and p14ARF. Blotting was with Rocklands Rabbit-anti-ZBP-89 antibody. For detection use Rocklands HRP conjugated Gt-anti-Rabbit IgG MX10 (611-103-122). See Bai and Merchant (2001) for additional details.
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