Anti-Alpha-1-Anti-trypsin Antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Alpha-1-Anti-trypsin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat)
Western Blot of Goat anti Alpha-1 anti-Trypsin antibody. Lane 1: Alpha-1 anti-Trypsin under reducing conditions. Load: 1 µg per lane. Primary antibody: Alpha-1 anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: DyLight 488 conjugated Donkey anti-goat secondary antibody at 1:10,000 for 45 min at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 55kDa for alpha-1 Anti-Trypsin. Other band(s): none.
Western Blot of Goat anti-Alpha-1-Anti-Trypsin antibody. Lane 1: Albumin depleted human serum. Load: ~10 µg per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody (p/n 605-4302) at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none
Western Blot of Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 at 30 min RT.
Western Blot of goat anti-Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green), Alpha 1 anti trypsin (red), and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 µg per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight(TM) 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight(TM) 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight(TM) 488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: Blocking Buffer for Fluorescent Western Blotting - MB-070 overnight at 4C
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