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AKT1 Antibody, IgG2b, Clone: [5E5.F5.D7], Mouse, Monoclonal

AKT1 Antibody, IgG2b, Clone: [5E5.F5.D7], Mouse, Monoclonal

Artikelnummer: ROC-200-301-J34

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Artikelname:	AKT1 Antibody, IgG2b, Clone: [5E5.F5.D7], Mouse, Monoclonal
Artikelnummer:	ROC-200-301-J34
Hersteller Artikelnummer:	200-301-J34
Alternativnummer:	ROC-200-301-J34
Hersteller:	Rockland Immunochemicals
Wirt:	Mouse
Kategorie:	Antikörper
Applikation:	ELISA, FC, WB
Spezies Reaktivität:	Human
Immunogen:	Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.
Konjugation:	Unconjugated
Alternative Synonym:	mouse anti-AKT1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

Klonalität:	Monoclonal
Konzentration:	1 mg/mL by UV absorbance at 280 nm
Klon-Bezeichnung:	[5E5.F5.D7]
Isotyp:	IgG2b
NCBI:	31749
UniProt:	P31749
Puffer:	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:	Liquid (sterile filtered)
Target-Kategorie:	Human
Antibody Type:	Primary Antibody

Application Verdünnung:	ELISA: 1:2,000 - 1:10,000, Flow Cytometry: User Optimized, IHC: 20 µg/mL, WB: 1:500-1:2000
Anwendungsbeschreibung:	Anti-AKT1 Antibody is tested in ELISA, Flow Cytometry, and western blotting. This antibody is suitable in immunohistochemistry. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or

	ELISA of Mouse Monoclonal anti-AKT1 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Mouse monoclonal anti-AKT1 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Mouse monoclonal anti-AKT1 antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100).

	Western Blot of Mouse Anti-AKT1 antibody. Lane 1: Molecular Weight Marker. Lane 2: LnCap lysate (p/n W09-001-GJ9). Lane 3: Jurkat lysate (p/n W09-001-370). Lane 4: MDA-MB 468 lysate (p/n W09-001-GG9). Load: 5 µg per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 56 kDa for AKT1.
	Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Lane 4: Molecular Weight Marker. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none.
	Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/mL for 20 min at 4C.

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