Anti-FOX3 Antibody was produced in mice by repeated immunizations with a recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli.
Konjugation:
Unconjugated
Alternative Synonym:
RNA binding protein fox-1 homolog 3, Fox-1 homolog C, NeuN, hexaribonucleotide binding protein 3
Klonalität:
Monoclonal
Konzentration:
titrated reagent Sufficient to run approximately 10 miniblots
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:20,000, IHC: 1:200-1:1000, IF Microscopy: 1:500, WB: 1:2000
Anwendungsbeschreibung:
Anti-FOX3 Antibody is tested for Western Blot, immunohistochemistry, and immunocytochemistry, and it is specific for the ~46/48 kDa FOX3 protein doublet. Specific conditions for reactivity should be optimized by the end user.
Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3 (p/n 200-301-X09, green, 1:1000) and Anti-MAP2 (red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
Western blot of Anti-FOX3 antibody. Rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3 (p/n 200-301-X09, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (1:1000, green) and nuclear staining was done with DAPI (blue).
Immunofluorescence of a section of rat cerebellum showing specific labeling of FOX3 (p/n 200-301-X09, 1:5000, red) and Neurofilament-L (1:2000, green). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3 labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat hippocampus showing specific labeling of FOX3 (p/n 200-301-X09, red) and UCHL1 (1:5000, green) in cell bodies and dendrites of neurons. The blue is DAPI staining of nuclear DNA.
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