Anti-MAPKAP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid synthetic peptide from near the N-terminus of human MAPKAP1.
Konjugation:
Unconjugated
Alternative Synonym:
MAPKAP1 Antibody, MIP1, SIN1, JC310, SIN1b, SIN1g, MIP1, Target of rapamycin complex 2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, TORC2 subunit MAPKAP1
Anti-MAPKAP1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 59 kDa in Western Blots of speci
Immunohistochemistry of MAPKAP1 antibody. Tissue: Human skeletal muscle tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: MAPKAP1 antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: MAPKAP1 is nuclear and occasionally cytoplasmic. Staining: MAPKAP1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of MAPKAP1 antibody. Lane A: Human skeletal muscle tissue lysate at 0.5 µg/mL. Lane B: Human skeletal muscle tissue lysate at 1 µg/mL. Lane C: Human skeletal muscle tissue lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 59.1 kDa, ~65 kDa for MAPKAP1. Other band(s): MAPKAP1 splice variants and isoforms.
Immunofluorescence Microscopy of MAPKAP1 antibody. Tissue: Human skeletal muscle cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: MAPKAP1 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MAPKAP1 as red fluorescent signal.
Western Blot of MAPKAP1 antibody. Lane A: Human skeletal muscle tissue lysate at 1 µg/mL . Lane B: Human skeletal muscle tissue lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 59.1 kDa, ~65 kDa for MAPKAP1. Other band(s): MAPKAP1 splice variants and isoforms.
Immunohistochemistry of MAPKAP1 antibody. Tissue: Human skeletal muscle tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: MAPKAP1 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: MAPKAP1 is nuclear and occasionally cytoplasmic. Staining: MAPKAP1 as precipitated red signal with hematoxylin purple nuclear counterstain.
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