Histone H3 [Dimethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 4 of human Histone H3.2.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ChIP: 2-5µg/million cells, IHC: 1:50, IF Microscopy: 1:50, WB: 1:500
Anwendungsbeschreibung:
Anti-Histone H3 [Dimethyl Lys4] antibody is tested in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. This antibody is useful in Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. E
Immunofluorescence of Rabbit Anti-Histone H3 [Dimethyl Lys4] Antibody. Tissue: C. elegans embryo lysate. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Dimethyl Lys4] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Dimethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [Dimethyl Lys4] is expressed in green and the nuclei are counterstained blue with DAPI.
Western Blot of Rabbit Anti-Histone H3 [Dimethyl Lys4] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys4] at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [Dimethyl Lys4] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 K4 Me3 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Western Blot of Rabbit Anti-Histone H3 [Dimethyl Lys4] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys4] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Dot Blot of Rabbit Histone H3 [Dimethyl Lys4] Antibody. Lane 1: K4. Lane 2: K4 Kme1. Lane 3: K4 Kme2. Lane 4: K4 Kme3. Lane 5: K4 KAc. Load: 1, 3, 10, and 25 picomoles of peptide. Primary antibody: Histone H3 [Dimethyl Lys4] antibody at 1:1000 for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
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