Histone H3 [Trimethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 9 of human Histone H3.2.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ChIP: 2-5µg/million cells, IHC: 1:1000, IF Microscopy: 1:1000, WB: 1:5000
Anwendungsbeschreibung:
Anti-Histone H3 [Trimethyl Lys9] antibody is tested by Western Blot, Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately
Dot Blot of Rabbit Histone H3 [Trimethyl Lys9] Antibody. Lane 1: K9 KMe1. Lane 2: K9 KMe2. Lane 3: K9 KMe3. Lane 4: K9. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Trimethyl Lys9] antibody at 1:1000 for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys9] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys9] at 1:5000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Immunofluorescence of Rabbit Anti-Histone H3 [Trimethyl Lys9] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Trimethyl Lys9] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Trimethyl Lys9] is nuclear and chromosomal. Staining: Histone H3 [Trimethyl Lys9] is expressed in green and the nuclei are counterstained with DAPI (blue).
Chromatin Immunoprecipitation of Anti Histone H3 [Trimethyl Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of Anti-Histone H3K9Me3 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys9] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys9] at 1:5000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
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