Histone H3 [ac Lys9, ac Lys14] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysines 9 and 14 of human Histone H3.2.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-Histone H3 Ac Lys9 / Ac Lys14 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9ac/K14ac
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ChIP: 2-5µg/million cells, IHC: 1:200, IF Microscopy: 1:200, WB: 1:500
Anwendungsbeschreibung:
Anti-Histone H3 [ac Lys9, ac Lys14] antibody is tested for Western Blot, Dot Blot, and Immunofluorescence. This antibody is useful for Chromatin Immunoprecipitation and Immunocytochemistry. Specific conditions for reactivity should be optimized by the en
ImmunoFluorescence of Rabbit Anti-Histone H3 K9ac/K14ac Antibody. Cells: HeLa Cells. Fixation: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Histone H3 [ac Lys9, ac Lys14] at 5µg/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight(TM)488 (p/n 611-141-122) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Actin Filament Stain: Texas Red - X Phallodin. Staining: (A) DAPI. (B) K9 AC/K14 AC + DyLight(TM)488. (C) Actin-X Phallodin. (D) Merge A+B. (E) Merge A+B+C. (F) Secondary Only Merge. Expected Location: Nuclear and Chromosomal.
Immunofluorescence of Rabbit Anti-Histone H3 [ac Lys9, ac Lys14] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [ac Lys9, ac Lys14] antibody at a 1:200 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [ac Lys9, ac Lys14] is nuclear and chromosomal. Staining: Histone H3 [ac Lys9, ac Lys14] is expressed in green, nuclei and actin are counterstained with Dapi (blue) and Phalloidin (red).
Western Blot of Rabbit Anti-Histone H3 [ac Lys9, ac Lys14] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys9, ac Lys14] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Dot Blot of Rabbit Histone H3 [K9ac, K14ac] Antibody. Lane 1: K9-K14. Lane 2: K9-KAc-K14-Kac. Lane 3: K9-K14-Kac. Lane 4: K9-Ac-K14. Load: 0.3, 1, and 3 µg of peptide. Primary antibody: Histone H3 [ac Lys9, ac Lys14] antibody for 45 min at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO A overnight at 4C.
Western Blot of Rabbit Anti-Histone H3 [ac Lys9, ac Lys14] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [ac Lys9, ac Lys14] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
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