Histone H3 K27me3/phospho S28 Antibody, Rabbit, Polyclonal

Artikelname:	Histone H3 K27me3/phospho S28 Antibody, Rabbit, Polyclonal
Artikelnummer:	ROC-600-401-I84
Hersteller Artikelnummer:	600-401-I84
Alternativnummer:	ROC-600-401-I84
Hersteller:	Rockland Immunochemicals
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ChIP, DOT, IF, Multiplex Assay, WB
Spezies Reaktivität:	C. elegans, Human, Mouse
Immunogen:	Histone H3 [Trimethyl Lys27, p Ser28] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic trimethylated/phosphorylated peptides surrounding Lysine 27 and Serine 28 of human Histone H3.
Konjugation:	Unconjugated
Alternative Synonym:	rabbit anti-Histone H3 trimethyl Lys18 pS28 antibody, H3.3B, H3pS28K18Me3, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3 K27me3/pS28

Klonalität:	Polyclonal
Konzentration:	0.66mg/mL by UV absorbance at 280 nm
NCBI:	126961
UniProt:	Q71DI3
Puffer:	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:	Liquid (sterile filtered)
Target-Kategorie:	Human
Antibody Type:	Primary Antibody

Application Verdünnung:	ChIP: 2-5µg/million cells, IHC: 1:2000, IF Microscopy: 1:2000, WB: 1 µg/mL
Anwendungsbeschreibung:	Anti-Histone H3 [Trimethyl Lys27, p Ser28] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band app

	Immunofluorescence of Rabbit Anti-Histone H3 [p Ser28, Trimethyl Lys27] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Ser28, Trimethyl Lys27] antibody at a 1:2000 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Ser28, Trimethyl Lys27] is nuclear and chromosomal. Staining: Histone H3 [p Ser28, Trimethyl Lys27] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

	Immunofluorescence of Rabbit Anti-Histone H3 [p Ser28, Trimethyl Lys27] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [p Ser28, Trimethyl Lys27] antibody at a 1:2000 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [p Ser28, Trimethyl Lys27] is nuclear and chromosomal. Staining: Histone H3 [p Ser28, Trimethyl Lys27] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
	Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys27, p Ser28] Antibody. Lane 1: HeLa histone prep. Lane 2: NIH-3T3 histone prep. Lane 3: C. elegans embryo cell lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys27, p Ser28] at 1 µg/ml for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
	Dot Blot of Rabbit Histone H3 [p Ser28, Trimethyl Lys27] Antibody. Lane 1: pS28/K27 unmodified. Lane 2: pS28 N-Term. Lane 3: p28 C-term. Lane 4: K27Me3. Lane 5: pS28/K27Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [p Ser28, Trimethyl Lys27] antibody at 1 µg/ml for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
	Chromatin Immunoprecipitation Rabbit Anti-Histone H3 [Trimethyl Lys27, p Ser28] Antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of Anti-Histone H3 K27me3pS28 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.