Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic dimethylated/dimethylated peptides surrounding Arginine 2 / Lysine 4 of human Histone H3.2.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ChIP: 2-5µg/million cells, IHC: 1:100, IF Microscopy: 1:100, WB: 1:500
Anwendungsbeschreibung:
Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~
Immunofluorescence of Rabbit Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Western Blot of Rabbit Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Lane 1: C. elegans embryo cell lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Chromatin Immunoprecipitation of Rabbit Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 R2me2s-K4me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Western Blot of Rabbit Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Dot Blot of Rabbit Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] Antibody. Lane 1: R2Me2. Lane 2: R2Me2/K4Me2. Lane 3: K4Me2. Load: 0.1, 1, 0.5, 5, and 10 picomoles of peptide. Primary antibody: Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] antibody at 1:1000 for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Final Antibody reactivity to R2Me2/K4Me2 seen in the right blot.
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