Histone H4 [p Ser1] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 1 of human Histone H4.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ChIP: 2-5µg/million cells, IHC: 1:50, IF Microscopy: 1:50, WB: 1:1000
Anwendungsbeschreibung:
Anti-Histone H4 [p Ser1] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kD
Immunofluorescence of Rabbit Anti-Histone H4 [p Ser1] Antibody. Tissue: Nonmitotic and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [p Ser1] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [p Ser1] is nuclear. Staining: Histone H4 [p Ser1] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Western Blot of Rabbit Anti-Histone H4 [p Ser1] Antibody. Lane 1: HeLa histone prep. Load: 30 µg per lane. Primary antibody: Histone H4 [p Ser1] at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~13 kDa. Other band(s): None.
Immunofluorescence of Rabbit Anti-Histone H4 [p Ser1] Antibody. Tissue: Nonmitotic, prophase, and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [p Ser1] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [p Ser1] is nuclear. Staining: Histone H4 [p Ser1] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Chromatin Immunoprecipitation of Rabbit Anti-Histone H4 [p Ser1] Antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of Anti-Histone H4 pS1 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
Dot Blot of Rabbit Histone H4 [p Ser1] Antibody. Lane 1: Histone H4, 1-15. Lane 2: Histone H4 pS1. Lane 3: Histone H4, 1-24. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 [p Ser1] antibody at 0.1 µg/ml for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
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