0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Human
Antibody Type:
Secondary Antibody
Application Verdünnung:
FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
Anwendungsbeschreibung:
Anti-Human IgG (H&L) DyLight 549 has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including
Properties of DyLight(TM) Fluorescent Dyes.
702 Peptides are printed in duplicates randomly distributed on the microarray. Control peptides (HA and FLAG controls) are located in a square surrounding the peptides of interest. As secondary antibody DyLightTM 549 conjugated goat anti-human IgG antibody and for the FLAG control peptide a mouse anti-FLAG-Cy3 antibody were used, microarrays were read using a Fujifilm Life Science FLA-5100 imaging system with a SHG 532nm (green) diode laser and an LPG filter. Fig e1. PMID:26894206.
DyLight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 549 conjugate. Anti-TNFa was detected using a DyLight(TM) 649 conjugate. The image was captured using the Typhoon(TM) 9410 Imaging System.
Western Blot of Goat anti-Human IgG Antibody DyLight(TM) 549 Conjugated. Lane 1: Human IgG. Load: 50 ng per lane. Secondary antibody: Human IgG (H&L) Antibody DyLight(TM) 549 Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa.
DyLight(TM) 549 Fluorescence Spectra.
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