0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Mouse
Antibody Type:
Secondary Antibody
Application Verdünnung:
FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
Anwendungsbeschreibung:
Anti-Mouse IgG DyLight 549 Antibody has been tested by ELISA, dot blot, and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multip
DyLight(TM) dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 549 conjugate. Anti-TNFa was detected using a DyLight(TM) 649 conjugate. The image was captured using the Typhoon(TM) 9410 Imaging System.
Western Blot of DyLight(TM) 549 conjugated Goat anti-Mouse IgG Pre-adsorbed secondary antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: DyLight(TM) 549 goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): none.
DyLight(TM) 549 Fluorescence Spectra
Properties of DyLight(TM) Conjugates.
Rockland Dylight 549 Goat Anti Mouse IgG antibody-Immunofluorescence Cell Type: A431 cells Fixation: 4% paraformaldehyde 10 min Permeabilization: 0.5% Triton X 30 min Primary Ab: 200-301-880 lot 28977 1:250 72 hours 4C Secondary Ab: 610-142-121 lot 21290 1:1000 Overnight 4C
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