0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Rat
Antibody Type:
Secondary Antibody
Application Verdünnung:
FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
Anwendungsbeschreibung:
Anti-Rat IgG DyLight(TM)488 has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight(TM) conjugate match the principle out
Western Blot of Dylight(TM) 488 conjugated Goat Anti-Rat IgG secondary antibody. Lane 1: Rat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Dylight(TM) 488 goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min t RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rat IgG. Other band(s): None.
Immunohistochemistry and immunocytochemistry of canine DEP domain-containing 1B (DEPDC1B). (B) DEPDC1B expression in a canine mammary gland tumor cell line CHM-p was assessed by immunocytochemical staining. CHM-p was fixed, stained with anti-canine DEPDC1B monoclonal antibody clone 4 or rat IgG2b, followed by DyLight 488-labelled anti-IgG antibody. DEPDC1B protein was visualized using fluorescence microscopy. Nuclei were stained with DAPI. Scale bar: 10µm. Fig 2. 32147621.
DyLight(TM) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight(TM) dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight(TM) 488 conjugate (green). Anti-Tubulin was detected using a DyLight(TM) 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
DyLight(TM) 488 Fluorescence Spectra
Properties of DyLight(TM) Fluorescent Dyes.
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