Chemiluminescent Western Blot Kit for use with Mouse Primary Antibody FemtoMax(TM)
Artikelnummer:
ROC-KCA002
- Bilder (5)
| Artikelname: | Chemiluminescent Western Blot Kit for use with Mouse Primary Antibody FemtoMax(TM) |
| Artikelnummer: | ROC-KCA002 |
| Hersteller Artikelnummer: | KCA002 |
| Alternativnummer: | ROC-KCA002 |
| Hersteller: | Rockland Immunochemicals |
| Kategorie: | Kits/Assays |
| Applikation: | WB |
| Spezies Reaktivität: | Mouse |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit |
| Application Verdünnung: | WB: 1:20,000 |
| Anwendungsbeschreibung: | Rockland Immunochemicals Chemiluminescent Western Blot Kit, namely our FemtoMax(TM) Chemiluminescent Substrate Kit for Western Blotting, combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our lumi |
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This product is assembled as a kit. See attached protocol or CofA for further details. |
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Chemiluminescent Western Blot Kit shows super sensitive s |
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Chemiluminescent Western Blot Kit shows super sensitive signal. Known amounts of GST were spiked into a HeLa lysate followed by separation by SDS-PAGE using a 4-20 % gradient gel. Proteins were transferred to a Protran B85 membrane for 1 hour at 100 mV. The membrane was blocked with 5% BLOTTO (p/n B501-0500) in TTBS for 1 h at 4 C. The blot was probed for 40 min with 1:1,000 dilution of Mab anti-GST (p/n 200-301-200) followed by HRP anti-Mouse IgG (p/n 610-103-121) at 1:10,000 dilution in blocking buffer for 1 h at 4C. |
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Flow Diagram for Chemiluminescent Western Blot Procedure |
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Rockland FemtoMax(TM) Chemiluminescent Western blot kits have reagents to run up to 30 mini blots at 7.5 x 8 cm2 |
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