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12 Epitope Tag Control Lysate (GST)

12 Epitope Tag Control Lysate (GST)

Artikelnummer: ROC-MB-302-0100

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Artikelname:	12 Epitope Tag Control Lysate (GST)
Artikelnummer:	ROC-MB-302-0100
Hersteller Artikelnummer:	MB-302-0100
Alternativnummer:	ROC-MB-302-0100
Hersteller:	Rockland Immunochemicals
Kategorie:	Zellen/Zellkultur
Applikation:	SDS-PAGE, WB
Konjugation:	Unconjugated
Alternative Synonym:	Flag-tag marker, GST-tag marker, HA-tag marker, 6XHIS-tag marker, T7-tag marker, cMyc-tag marker, V5-tag marker, S-tag marker, HSV-tag marker, VSV-tag marker, Glu-Glu-tag marker, E-tag marker, Lysate marker, Protein Marker, Loading control, epitope tag marker, positive control marker

Konzentration:	1.0 mg/mL by modified Lowry assay
Puffer:	1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Formulierung:	Liquid

Application Verdünnung:	WB: User Optimized
Anwendungsbeschreibung:	This 12 Epitope Tag Protein Marker Lysate has been tested in SDS-PAGE and western blot and is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly us

	Western Blot of GST tag- 12 epitope protein marker lysate. Lane 1: 7µL load of MB-210-0500 molecular weight marker. Lane 2: 5µg load of 12 epitope GST tag protein marker. Antibody: Incubate for 1hr at RT. Lane 2a: Mouse anti-Flag DyLight488 (p/n 200-341-383) at 1:40,000. Lane 2b: Mouse anti-6xHis DyLight488 (p/n 200-341-382) at 1:40,000. Lane 2c: Mouse anti-GST DyLight649 (p/n 200-343-200) at 1:5,000. Lane 2d: Rabbit anti-cMyc DyLight488 (p/n 600-441-381) at 1:10,000. Lane 2e: Rabbit anti-HA DyLight649 (p/n 600-443-384) at 1:10,000.

	Western Blot of GST tag- 12 epitope protein marker lysate. Lane 1: 7µL load of MB-210-0500 molecular weight marker. Lane 2: 10µg load of 12 epitope GST tag protein marker. Antibody: Incubate for 1hr at RT. Lane 2a: Mouse anti-Flag DyLight488 (p/n 200-341-383) at 1:40,000. Lane 2b: Mouse anti-6xHis DyLight488 (p/n 200-341-382) at 1:40,000. Lane 2c: Mouse anti-GST DyLight649 (p/n 200-343-200) at 1:5,000. Lane 2d: Rabbit anti-cMyc DyLight488 (p/n 600-441-381) at 1:10,000. Lane 2e: Rabbit anti-HA DyLight649 (p/n 600-443-384) at 1:10,000.
	SDS-Page of 12 Epitope GST Tag Protein Lysate. Lane 1: Opal Molecular Weight MB-210-0500. Lane 2: 0.5µg lysate. Lane 3: 1.0µg lysate. Lane 4: 2.0µg lysate. Lane 5: 3.0µg lysate. Lane 6: 5.0µg lysate.

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